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Infection and Immunity, December 2005, p. 8411-8417, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8411-8417.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Two Non-Locus of Enterocyte Effacement-Encoded Type III-Translocated Effectors, NleC and NleD, in Attaching and Effacing Pathogens

Olivier Marchés,1 Siouxsie Wiles,1 Francis Dziva,2 Roberto M. La Ragione,3 Stephanie Schüller,4 Angus Best,3 Alan D. Phillips,4 Elizabeth L. Hartland,5 Martin J. Woodward,3 Mark P. Stevens,2 and Gad Frankel1*

Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom,1 Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom,2 Department of Food & Environmental Safety, Veterinary Laboratories Agency (Defra), Woodham Lane, Addlestone, Surrey KT15 3NB, United Kingdom,3 Centre for Paediatric Gastroenterology, Royal Free and University College Medical School, London, United Kingdom,4 Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia5

Received 20 April 2005/ Returned for modification 16 June 2005/ Accepted 17 August 2005

Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.

* Corresponding author. Mailing address: CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom. Phone: 44 (0) 20 7594 5253. Fax: 44 (0) 20 7594 3069. E-mail: g.frankel{at}imperial.ac.uk.

Editor: D. L. Burns

Infection and Immunity, December 2005, p. 8411-8417, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8411-8417.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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