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Infection and Immunity, February 2005, p. 1006-1013, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1006-1013.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Differential PsaA-, PspA-, PspC-, and PdB-Specific Immune Responses in a Mouse Model of Pneumococcal Carriage

Ravichandran Palaniappan,1,2 Shailesh Singh,2 Udai P. Singh,2 Senthil Kumar K. Sakthivel,2 Edwin W. Ades,3 David E. Briles,4 Susan K. Hollingshead,4 James C. Paton,5 Jacquelyn S. Sampson,3 and James W. Lillard Jr.2,4*

Department of Pharmaceutical Sciences, Southern School of Pharmacy, Mercer University,1 Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine,2 Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,3 Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama,4 School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia5

Received 26 August 2004/ Returned for modification 20 September 2004/ Accepted 20 October 2004

Larger numbers of pneumococci were detected in the nasal tract compared to the lung, cervical lymph nodes, and spleen 1, 2, 4, 7, 14, and 21 days after nasal challenge with Streptococcus pneumoniae strain EF3030. In this mouse model of pneumococcal carriage, peripheral S. pneumoniae pneumococcal surface adhesin A (PsaA)-specific humoral responses (immunoglobulin G2a [IgG2a] >> IgG1 = IgG2b > IgG3) were significantly higher than pneumococcal surface protein A (PspA)-specific, genetic toxoid derivative of pneumolysin (PdB)-specific, or pneumococcal surface protein C (PspC)-specific serum antibody levels. However, PspA-specific mucosal IgA antibody levels were significantly higher than those against PsaA, PdB, and PspC. In general, both PsaA- and PspA-specific lung-, cervical lymph node-, nasal tract-, and spleen-derived CD4+ T-cell cytokine (interleukin-4, interleukin-6, granulocyte-macrophage colony-stimulating factor, gamma interferon, and tumor necrosis factor alpha) and proliferative responses were higher than those for either PspC or PdB. Taken together, these findings suggest that PsaA- and PspA-specific mucosal responses as well as systemic humoral and T helper cell cytokine responses are predominantly yet differentially induced during pneumococcal carriage.

* Corresponding author. Mailing address: Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310-1495. Phone: (404) 752-1581. Fax: (404) 752-1179. E-mail: lillard{at}msm.edu.

Editor: J. T. Barbieri

Infection and Immunity, February 2005, p. 1006-1013, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1006-1013.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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