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Infection and Immunity, February 2005, p. 1052-1060, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1052-1060.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin

Anders Krarup,1 Uffe B. Skov Sørensen,1 Misao Matsushita,2 Jens C. Jensenius,1 and Steffen Thiel1*

Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark,1 Institute of Glycotechnology, Department of Applied Biochemistry, Tokai University, Kanagawa, Japan2

Received 30 June 2004/ Returned for modification 17 August 2004/ Accepted 21 September 2004

Mannan-binding lectin (MBL), L-ficolin, and H-ficolin are pattern recognition molecules of the innate immune system. We investigated their ability to bind to different serotypes and noncapsulated variants of two gram-positive bacterial species, Streptococcus pneumoniae and Staphylococcus aureus. MBL did not bind to capsulated S. aureus or capsulated S. pneumoniae but did bind to a noncapsulated S. aureus variant (Wood). L-ficolin bound to some capsulated S. aureus serotypes (serotypes 1, 8, 9, 11, and 12) and capsulated S. pneumoniae serotypes (11A, 11D, and 11F) but not to noncapsulated strains. H-ficolin did not bind to any of the S. pneumoniae and S. aureus serotypes included in this study but did bind to one strain of Aerococcus viridans. The concentrations of the three proteins in 97 plasma samples were estimated. The median concentrations were 0.8 µg per ml for MBL, 3.3 µg per ml for L-ficolin, and 18.4 µg per ml for H-ficolin.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, Aarhus C DK-8000, Denmark. Phone: 45 89421776. Fax: 45 86196128. E-mail: st{at}microbiology.au.dk.

Editor: T. R. Kozel

Infection and Immunity, February 2005, p. 1052-1060, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1052-1060.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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