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Infection and Immunity, February 2005, p. 1061-1068, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1061-1068.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of Contact Lens Wear, Bacterial Flora, and Mannose-Induced Pathogenic Protease in the Pathogenesis of Amoebic Keratitis

Hassan Alizadeh, Sudha Neelam, Michael Hurt, and Jerry Y. Niederkorn*

Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas

Received 3 August 2004/ Returned for modification 12 September 2004/ Accepted 8 October 2004

The ocular surface is continuously exposed to potential pathogens, including free-living amoebae. Acanthamoeba species are among the most ubiquitous amoebae, yet Acanthamoeba keratitis is remarkably rare. The pathogenesis of Acanthamoeba keratitis is a complex, sequential process. Here we show that Acanthamoeba keratitis is profoundly affected by mannosylated proteins on the ocular surface, which stimulate the amoebae to elaborate a 133-kDa pathogenic protease. The mannose-induced protease (MIP133) mediates apoptosis of the corneal epithelium, facilitates corneal invasion, and degrades the corneal stroma. We show that contact lens wear upregulates mannosylated proteins on the corneal epithelium, stimulates MIP133 secretion, and exacerbates corneal disease. Corynebacterium xerosis, a constituent of the ocular flora, contains large amounts of mannose and is associated with Acanthamoeba keratitis. The present results show that amoebae exposed to C. xerosis produce increased amounts of MIP133 and more severe corneal disease. Oral immunization with MIP133 mitigates Acanthamoeba keratitis and demonstrates the feasibility of antidisease vaccines for pathogens that resist immune elimination.

* Corresponding author. Mailing address: Department of Ophthalmology, U.T. Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9057. Phone: (214) 648-3829. Fax: (214) 648-9061. E-mail: jerry.niederkorn{at}utsouthwestern.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, February 2005, p. 1061-1068, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1061-1068.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Clarke, D. W., Alizadeh, H., Niederkorn, J. Y. (2006). Intracorneal Instillation of Latex Beads Induces Macrophage-Dependent Protection against Acanthamoeba Keratitis. IOVS 47: 4917-4925 [Abstract] [Full Text]  
  • Garate, M., Marchant, J., Cubillos, I., Cao, Z., Khan, N. A., Panjwani, N. (2006). In vitro pathogenicity of acanthamoeba is associated with the expression of the mannose-binding protein.. IOVS 47: 1056-1062 [Abstract] [Full Text]  


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