This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rivera, J.
Right arrow Articles by Casadevall, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rivera, J.
Right arrow Articles by Casadevall, A.

 Previous Article  |  Next Article 

Infection and Immunity, February 2005, p. 1141-1150, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1141-1150.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Antibody-Mediated Protection against Cryptococcus neoformans Pulmonary Infection Is Dependent on B Cells

Johanna Rivera,1 Oscar Zaragoza,1 and Arturo Casadevall1,2*

Department of Microbiology and Immunology,1 Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York2

Received 21 May 2004/ Returned for modification 30 June 2004/ Accepted 31 October 2004

The pathogenesis of pulmonary Cryptococcus neoformans infection and the efficacy of passive immunoglobulin G1 (IgG1) administration were investigated in B-cell-deficient and C57BL/6J mice. C57BL/6J mice lived longer than B-cell-deficient mice after both intratracheal and intravenous infections. Administration of IgG1 prior to infection prolonged the survival of C57BL/6J mice but had no effect on the survival or numbers of CFU in the lungs of B-cell-deficient mice. C. neoformans infection in B-cell-deficient mice resulted in significantly higher levels of gamma interferon (IFN-{gamma}), monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-1{alpha} (MIP-1{alpha}) than in C57BL/6J mice. IgG1 administration reduced IFN-{gamma} and MCP-1 levels in C57BL/6J mice but not in B-cell-deficient mice. In addition, compared to its effect in C57BL/6J mice, C. neoformans infection in FcR{gamma}III-deficient, athymic, and SCID mice significantly increased IFN-{gamma} and MCP-1 levels. IgG1 administration was associated with reduced IFN-{gamma} levels in C57BL/6J mice but not in FcR{gamma}III-deficient, athymic, and SCID mice. These observations suggest that IgG1-mediated protection in this system is a consequence of alterations in the inflammatory response that translate into less damage to the host without directly reducing the fungal burden. For hosts with impaired immunities, the ineffectiveness of passive antibody (Ab) may reflect an inability to down-regulate inflammation and avoid self-damage. The results indicate an important role for B cells in host defense against C. neoformans infection and demonstrate a surprising dependence of Ab-mediated protection on B cells in this system.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-3730. Fax: (718) 430-8701. E-mail: casadeva{at}aecom.yu.edu.

Editor: T. R. Kozel

Infection and Immunity, February 2005, p. 1141-1150, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1141-1150.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Buendia, A. J., Ortega, N., Caro, M. R., Del Rio, L., Gallego, M. C., Sanchez, J., Navarro, J. A., Cuello, F., Salinas, J. (2009). B Cells Are Essential for Moderating the Inflammatory Response and Controlling Bacterial Multiplication in a Mouse Model of Vaccination against Chlamydophila abortus Infection. Infect. Immun. 77: 4868-4876 [Abstract] [Full Text]  
  • Guillot, L., Carroll, S. F., Homer, R., Qureshi, S. T. (2008). Enhanced Innate Immune Responsiveness to Pulmonary Cryptococcus neoformans Infection Is Associated with Resistance to Progressive Infection. Infect. Immun. 76: 4745-4756 [Abstract] [Full Text]  
  • Lazar-Molnar, E., Gacser, A., Freeman, G. J., Almo, S. C., Nathenson, S. G., Nosanchuk, J. D. (2008). The PD-1/PD-L costimulatory pathway critically affects host resistance to the pathogenic fungus Histoplasma capsulatum. Proc. Natl. Acad. Sci. USA 105: 2658-2663 [Abstract] [Full Text]  
  • Rodrigues, M. L., Shi, L., Barreto-Bergter, E., Nimrichter, L., Farias, S. E., Rodrigues, E. G., Travassos, L. R., Nosanchuk, J. D. (2007). Monoclonal Antibody to Fungal Glucosylceramide Protects Mice against Lethal Cryptococcus neoformans Infection. CVI 14: 1372-1376 [Abstract] [Full Text]  
  • Zaragoza, O., Alvarez, M., Telzak, A., Rivera, J., Casadevall, A. (2007). The Relative Susceptibility of Mouse Strains to Pulmonary Cryptococcus neoformans Infection Is Associated with Pleiotropic Differences in the Immune Response. Infect. Immun. 75: 2729-2739 [Abstract] [Full Text]  
  • Wozniak, K. L., Vyas, J. M., Levitz, S. M. (2006). In Vivo Role of Dendritic Cells in a Murine Model of Pulmonary Cryptococcosis. Infect. Immun. 74: 3817-3824 [Abstract] [Full Text]  
  • Ghosn, E. E. B., Russo, M., Almeida, S. R. (2006). Nitric oxide-dependent killing of Cryptococcus neoformans by B-1-derived mononuclear phagocyte. J. Leukoc. Biol. 80: 36-44 [Abstract] [Full Text]  
  • Yager, E., Bitsaktsis, C., Nandi, B., McBride, J. W., Winslow, G. (2005). Essential Role for Humoral Immunity during Ehrlichia Infection in Immunocompetent Mice. Infect. Immun. 73: 8009-8016 [Abstract] [Full Text]  
  • Subramaniam, K., French, N., Pirofski, L.-a. (2005). Cryptococcus neoformans-Reactive and Total Immunoglobulin Profiles of Human Immunodeficiency Virus-Infected and Uninfected Ugandans. CVI 12: 1168-1176 [Abstract] [Full Text]  
  • Arora, S., Hernandez, Y., Erb-Downward, J. R., McDonald, R. A., Toews, G. B., Huffnagle, G. B. (2005). Role of IFN-{gamma} in Regulating T2 Immunity and the Development of Alternatively Activated Macrophages during Allergic Bronchopulmonary Mycosis. J. Immunol. 174: 6346-6356 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»