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Long-Term Protection against Tuberculosis following Vaccination with a Severely Attenuated Double Lysine and Pantothenate Auxotroph of Mycobacterium tuberculosis

Vasan K. Sambandamurthy,^1,2, Steven C. Derrick,^3, Kripa V. Jalapathy,² Bing Chen,^1,2 Robert G. Russell,⁴ Sheldon L. Morris,³ and William R. Jacobs Jr.^1,2*

Howard Hughes Medical Institute,¹ Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York,² Department of Pathology, Lombardi Cancer Center, Georgetown University, Washington, D.C.,⁴ Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland³

Received 6 August 2004/ Accepted 20 September 2004

We report the safety and immunogenicity of a double lysine and pantothenate auxotroph of Mycobacterium tuberculosis in mice. The lysA panCD mutant is completely attenuated in immunocompromised SCID and gamma interferon knockout mice yet induces short-term and long-term protection in immunocompetent and CD4-deficient mice following single-dose subcutaneous vaccination.

Editor: A. D. O'Brien

V. K. Sambandamurthy and S. C. Derrick contributed equally to this work.

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