This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wing, H. J. Articles by Goldberg, M. B. Search for Related Content PubMed PubMed Citation Articles by Wing, H. J. Articles by Goldberg, M. B.

Modulation of an Outer Membrane Protease Contributes to the Virulence Defect of Shigella flexneri Strains Carrying a Mutation in the virK Locus

Helen J. Wing ,^, Seth R. Goldman, Shabeen Ally, and Marcia B. Goldberg^*

Infectious Disease Division, Massachusetts General Hospital, Boston, Massachusetts

Received 9 July 2004/ Returned for modification 16 September 2004/ Accepted 20 October 2004

The Shigella actin assembly protein IcsA is removed from the bacterial surface by the protease IcsP. We show that decreased intracellular spreading of virK::Tn10 mutants is due in part to significant increases in IcsP and IcsP-mediated cleavage of IcsA and that IcsP expression is a critical determinant of Shigella virulence.

Editor: J. N. Weiser

H.J.W. and S.R.G. contributed equally to this work.

Present address: Department of Biological Sciences, University of Nevada-Las Vegas, Las Vegas.