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Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to D-Mannose Receptors

William R. Schwan,^1* Michael T. Beck,^1, Scott J. Hultgren,² Jerry Pinkner,² Nathan L. Woolever,^1, and Thomas Larson¹

University of Wisconsin-La Crosse, La Crosse, Wisconsin,¹ Washington University, St. Louis, Missouri²

Received 10 August 2004/ Returned for modification 17 September 2004/ Accepted 30 September 2004

A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to D-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.

Editor: D. L. Burns

Present address: University of Minnesota, Minneapolis, Minn.

Present address: State Lab of Hygiene, Madison, Wis.

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