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Infection and Immunity, February 2005, p. 1260-1264, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1260-1264.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Type IV Pilin, PilA, Contributes to Adherence of Burkholderia pseudomallei and Virulence In Vivo

Angela E. Essex-Lopresti,1,{dagger}* Justin A. Boddey,2,{dagger} Richard Thomas,1 Martin P. Smith,1 M. Gill Hartley,1 Timothy Atkins,1 Nat F. Brown,2,{ddagger} Chuk Hai Tsang,1 Ian R. A. Peak,2 Jim Hill,1 Ifor R. Beacham,2 and Richard W. Titball1

Dstl Porton Down, Salisbury, Wiltshire, United Kingdom,1 Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia2

Received 28 June 2004/ Returned for modification 28 July 2004/ Accepted 30 September 2004

The Burkholderia pseudomallei K96243 genome contains multiple type IV pilin-associated loci, including one encoding a putative pilus structural protein (pilA). A pilA deletion mutant has reduced adherence to human epithelial cells and is less virulent in the nematode model of virulence and the murine model of melioidosis, suggesting a role for type IV pili in B. pseudomallei virulence.

* Corresponding author. Mailing address: Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom. Phone: (44) 01980 613179. Fax: (44) 01980 614307. E-mail: aeelopresti{at}dstl.gov.uk.

Editor: J. B. Bliska

{dagger} A.E.E.-L. and J.A.B. contributed equally to the work described in this paper.

{ddagger} Present address: Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, Canada.

Infection and Immunity, February 2005, p. 1260-1264, Vol. 73, No. 2
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.2.1260-1264.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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