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A Type IV Pilin, PilA, Contributes to Adherence of Burkholderia pseudomallei and Virulence In Vivo

Angela E. Essex-Lopresti,^1,* Justin A. Boddey,^2, Richard Thomas,¹ Martin P. Smith,¹ M. Gill Hartley,¹ Timothy Atkins,¹ Nat F. Brown,^2, Chuk Hai Tsang,¹ Ian R. A. Peak,² Jim Hill,¹ Ifor R. Beacham,² and Richard W. Titball¹

Dstl Porton Down, Salisbury, Wiltshire, United Kingdom,¹ Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia²

Received 28 June 2004/ Returned for modification 28 July 2004/ Accepted 30 September 2004

The Burkholderia pseudomallei K96243 genome contains multiple type IV pilin-associated loci, including one encoding a putative pilus structural protein (pilA). A pilA deletion mutant has reduced adherence to human epithelial cells and is less virulent in the nematode model of virulence and the murine model of melioidosis, suggesting a role for type IV pili in B. pseudomallei virulence.

Editor: J. B. Bliska

A.E.E.-L. and J.A.B. contributed equally to the work described in this paper.

Present address: Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, Canada.

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