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Infection and Immunity, March 2005, p. 1321-1329, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1321-1329.2005

Identification of Vaccine Candidate Peptides in the NcSRS2 Surface Protein of Neospora caninum by Using CD4⁺ Cytotoxic T Lymphocytes and Gamma Interferon-Secreting T Lymphocytes of Infected Holstein Cattle

Department of Veterinary Microbiology and Pathology, Washington State University,¹ Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington²

Received 25 May 2004/ Returned for modification 14 July 2004/ Accepted 3 November 2004

Previously, our laboratory showed that Holstein cattle experimentally infected with Neospora caninum develop parasite-specific CD4⁺ cytotoxic T lymphocytes (CTL) that lyse infected, autologous target cells through a perforin-granzyme pathway. To identify specific parasite antigens inducing bovine CTL and helper T-lymphocyte responses for vaccine development against bovine neosporosis, the tachyzoite major surface proteins NcSAG1 and NcSRS2 were targeted. In whole tachyzoite antigen-expanded bovine T-lymphocyte lines, recombinant NcSRS2 induced potent memory CD4⁺- and CD8⁺-T-lymphocyte activation, as indicated by proliferation and gamma interferon (IFN-) secretion, while recombinant NcSAG1 induced a minimal memory response. Subsequently, T-lymphocyte epitope-bearing peptides of NcSRS2 were mapped by using overlapping peptides covering the entire NcSRS2 sequence. Four experimentally infected cattle with six different major histocompatibility complex (MHC) class II haplotypes were the source of immune cells used to identify NcSRS2 peptides presented by Holstein MHC haplotypes. NcSRS2 peptides were mapped by using IFN- secretion by rNcSRS2-stimulated, short-term T-lymphocyte cell lines, IFN- enzyme-linked immunospot (ELISPOT) assay with peripheral blood mononuclear cells, and ⁵¹Cr release cytotoxicity assay of rNcSRS2-stimulated effector cells. Four N. caninum-infected Holstein cattle developed NcSRS2 peptide-specific T lymphocytes detected ex vivo in peripheral blood by IFN- ELISPOT and in vitro by measuring T-lymphocyte IFN- production and cytotoxicity. An immunodominant region of NcSRS2 spanning amino acids 133 to 155 was recognized by CD4⁺ T lymphocytes from the four cattle. These findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences or peptides for induction of NcSRS2 peptide-specific CTL and IFN--secreting T lymphocytes in cattle with varied MHC genotypes.

Editor: J. F. Urban, Jr.

This article has been cited by other articles:

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