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Infection and Immunity, March 2005, p. 1399-1403, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1399-1403.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Broad Degradation of Proapoptotic Proteins with the Conserved Bcl-2 Homology Domain 3 during Infection with Chlamydia trachomatis

Songmin Ying,1 Barbara M. Seiffert,1 Georg Häcker,1* and Silke F. Fischer1

Institute for Medical Microbiology, Technische Universität München, Munich, Germany1

Received 22 September 2004/ Returned for modification 1 November 2004/ Accepted 8 November 2004

Chlamydiae are obligate intracellular bacteria that can inhibit apoptosis of their host cell. As shown recently, this inhibition is in part explained by the proteolytic degradation of the proapoptotic Bcl-2 family members (BH3-only proteins) Bim, Puma, and Bad upon chlamydial infection. In this study, we further explore this antiapoptotic mechanism. In cells infected with a Chlamydia trachomatis L2 strain, Bim, Puma, and Bad were degraded with similar kinetics, and the degradation of all three was blocked by inhibition of the proteasome. Furthermore, the BH3-only proteins Bmf, Noxa, and tBid were also targeted by chlamydial infection. The constitutively expressed Bmf disappeared during infection. When Noxa was experimentally induced, the levels were also reduced by infection with C. trachomatis. In death-receptor-induced apoptosis, cleaved and activated tBid was degraded, and this destruction was also prevented by inhibition of the proteasome. These results show that chlamydial infection leads to a broad degradation of BH3-only proteins. This loss of proapoptotic factors can explain the almost general protection of infected cells against apoptotic stimuli.

* Corresponding author. Mailing address: Institute for Medical Microbiology, Technische Universität München, Trogerstr. 9, D-81675 Munich, Germany. Phone: 49-89-4140-4121. Fax: 49-89-4140-4868. E-mail: hacker{at}lrz.tum.de.

Editor: D. L. Burns

Infection and Immunity, March 2005, p. 1399-1403, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1399-1403.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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