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Infection and Immunity, March 2005, p. 1475-1481, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1475-1481.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Efficacy of Vaccination of Calves against Hemorrhagic Septicemia with a Live aroA Derivative of Pasteurella multocida B:2 by Two Different Routes of Administration

J. Christopher Hodgson,1 Anna Finucane,2 Mark P. Dagleish,1 Saeed Ataei,2 Roger Parton,2 and John G. Coote2*

Moredun Research Institute, International Research Centre, Bush Loan, Penicuik, Midlothian,1 Infection and Immunity Division, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom2

Received 16 July 2004/ Returned for modification 29 September 2004/ Accepted 12 November 2004

Two groups of four calves each were immunized either intramuscularly (i.m. vaccinated) or intranasally (i.n. vaccinated) at 2 and 6 weeks of age with ca. 109 CFU of a derivative of P. multocida serotype B:2 strain 85020 containing a deletion in the aroA gene (strain JRMT12). Both groups of calves and three unvaccinated control calves were challenged subcutaneously at 8 weeks of age with ca. 107 CFU of the wild-type 85020 strain. The first and second vaccinations caused a significant pyrexia and increase in the mean demeanor score (P < 0.05) in i.m. but not i.n. vaccinated calves. Serum agglutinating activity against whole cells of P. multocida strain 85020 and immunoglobulin G antibody concentrations increased after the second vaccination in i.m. but not in i.n. vaccinated animals, and this difference was statistically significant (P < 0.05). Concentrations of serum amyloid A (SAA) increased significantly 3 h after both the primary (P < 0.05) and booster (P < 0.001) i.m. vaccinations, but not in i.n. vaccinated calves. All four i.m. vaccinated calves were solidly immune to challenge with wild-type P. multocida B:2. However, the mean rectal temperatures, demeanor scores, and serum SAA concentrations of i.n. vaccinated and control calves increased significantly (P < 0.01). Three i.n. vaccinated and two control calves were killed for humane reasons within 14 h postchallenge, and postmortem examination revealed pathological lesions consistent with hemorrhagic septicemia. These data showed that the aroA mutant strain, given i.m. as two doses 4 weeks apart, acted as an effective live-attenuated vaccine strain to protect calves against challenge with the virulent parent strain.

* Corresponding author. Mailing address: Infection and Immunity Division, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 44 (0) 141 330 5845. Fax: 44 (0) 141 330 4600. E-mail: j.coote{at}bio.gla.ac.uk.

Editor: J. T. Barbieri

Infection and Immunity, March 2005, p. 1475-1481, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1475-1481.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dagleish, M. P., Hodgson, J. C., Ataei, S., Finucane, A., Finlayson, J., Sales, J., Parton, R., Coote, J. G. (2007). Safety and Protective Efficacy of Intramuscular Vaccination with a Live aroA Derivative of Pasteurella multocida B:2 against Experimental Hemorrhagic Septicemia in Calves. Infect. Immun. 75: 5837-5844 [Abstract] [Full Text]  


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