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Infection and Immunity, March 2005, p. 1656-1663, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1656-1663.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison between Candida albicans Agglutinin-Like Sequence Gene Expression Patterns in Human Clinical Specimens and Models of Vaginal Candidiasis

Georgina Cheng,1 Karen Wozniak,2 Matthew A. Wallig,1 Paul L. Fidel Jr.,2 Suzanne R. Trupin,3 and Lois L. Hoyer1*

Department of Veterinary Pathobiology,1 Department of Obstetrics and Gynecology, University of Illinois, Urbana, Illinois,3 Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana2

Received 26 August 2004/ Returned for modification 13 September 2004/ Accepted 22 October 2004

Expression of the eight genes in the Candida albicans agglutinin-like sequence (ALS) family was studied by reverse transcription-PCR of RNA isolated from clinical vaginal fluid specimens and vaginal candidiasis model systems. Although expression of all ALS genes was detected across the set of clinical specimens, ALS1, ALS2, ALS3, and ALS9 transcripts were detected most frequently, and expression of ALS4 and ALS5 was detected least frequently. Laboratory strain 3153A and two C. albicans strains isolated from the clinical specimens were studied using two models of vaginal candidiasis to determine how closely these models mimicked the clinical specimens at the level of gene expression. ALS gene expression patterns in a murine vaginitis model were identical to those from the clinical specimens. Expression of more ALS genes was detected in specimens collected 7 days after infection compared to those collected at 4 days. Similar patterns of ALS gene expression were observed when the three C. albicans strains were tested in the reconstituted human vaginal epithelium model. In this model, expression of ALS4, ALS5, ALS6, and ALS7 was least frequently detected. Negative or weakened signals for ALS4 expression were observed at early time points, suggesting that ALS4 expression, which was strong in the inoculum cells, was down-regulated upon contact of C. albicans with vaginal epithelial cells in this model. The data presented here support the conclusion of host-site-specific influences on ALS gene expression and validate the use of the experimental models for evaluating the phenotype of als/als mutant strains.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, University of Illinois, Urbana, 2522 VMBSB, 2001 S. Lincoln Avenue, Urbana, IL 61802. Phone: (217) 333-5043. Fax: (217) 244-7421. E-mail: lhoyer{at}uiuc.edu.

Editor: T. R. Kozel

Infection and Immunity, March 2005, p. 1656-1663, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1656-1663.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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