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Infection and Immunity, March 2005, p. 1714-1722, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1714-1722.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Salmonella enterica Serovar Typhimurium Infection of Dendritic Cells Leads to Functionally Increased Expression of the Macrophage-Derived Chemokine

Guo Fu,1 Odilia L. C. Wijburg,1,2 Paul U. Cameron,1 Jason D. Price,1 and Richard A Strugnell1,2*

CRC for Vaccine Technology and Australian Bacterial Pathogenesis Program, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria,1 Australia2

Received 20 February 2004/ Returned for modification 21 April 2004/ Accepted 12 November 2004

Gene expression in murine dendritic cells (DCs) infected with green fluorescent protein-expressing Salmonella enterica serovar Typhimurium BRD509 was studied by mRNA differential display. Infected DCs were sorted from uninfected cells by flow cytometry. The mRNA expression patterns of infected and uninfected cells revealed a number of differentially expressed transcripts, which included the macrophage-derived chemokine (MDC). Up-regulation of MDC transcription in infected DCs was confirmed by Northern blotting, and the kinetics of MDC expression was examined by real-time reverse transcription-PCR, with which 31- and 150-fold increases were detected at 2 and 6 h postinfection, respectively. The increased release by DCs of MDC into culture media was detected by an enzyme-linked immunosorbent assay. The biological activity of MDC was investigated in in vitro and in vivo assays. In vitro, supernatants from S. enterica serovar Typhimurium-infected DCs were chemoattractive to T cells, and neutralization of MDC in these supernatants inhibited T-cell migration. Passive transfer of anti-MDC antibody to mice infected with BRD509 revealed that neither growth of the bacterium nor resistance of the mice to reinfection was affected and that in vivo inhibition of MDC did not affect T-cell responses, as measured by the gamma interferon ELISPOT method 3 days after challenge infection.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia. Phone: 61 3 8344 5712. Fax: 61 3 9347 1540. E-mail: rastru{at}unimelb.edu.au.

Editor: S. H. E. Kaufmann

Infection and Immunity, March 2005, p. 1714-1722, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1714-1722.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • DePaolo, R. W., Lathan, R., Rollins, B. J., Karpus, W. J. (2005). The Chemokine CCL2 Is Required for Control of Murine Gastric Salmonella enterica Infection. Infect. Immun. 73: 6514-6522 [Abstract] [Full Text]  


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