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Infection and Immunity, March 2005, p. 1735-1743, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1735-1743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Enterohemorrhagic Escherichia coli O26:H– Genes Required for Intestinal Colonization in Calves

Pauline M. van Diemen,1 Francis Dziva,1 Mark P. Stevens,1 and Timothy S. Wallis1*

Mammalian Enteric Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Berkshire, United Kingdom1

Received 7 June 2004/ Returned for modification 14 September 2004/ Accepted 28 September 2004

Enterohemorrhagic Escherichia coli (EHEC) infections in humans are an important public health problem and are commonly acquired via contact with ruminant feces. The serogroups that are predominantly associated with human infection in the United States and Europe are O157 and O26. Serotypes O157:H7 and O26:H– differ in their virulence and tissue tropism in calves and therefore may colonize calves by distinct mechanisms. The mechanisms underlying EHEC intestinal colonization and pathogenesis are poorly understood. Signature-tagged mutagenesis was used to identify 59 genes of EHEC O26:H– that are required for the intestinal colonization of calves. Our results indicate important roles for locus of enterocyte effacement (LEE)-encoded type III secreted proteins in intestinal colonization. In addition, colonization is facilitated by cytotoxins, putative type III secreted proteins unlinked to the LEE, a putative fimbrial operon, and numerous genes involved in central metabolism and transport and genes of unknown function. Our data also imply that the elaboration of type I fimbriae by EHEC O26:H– is disadvantageous for persistence within the bovine intestines. These observations have important implications for the design of vaccines to control these important zoonotic pathogens.

* Corresponding author. Mailing address: Mammalian Enteric Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 578411. Fax: 44 (0)1635 577243. E-mail: timothy.wallis{at}bbsrc.ac.uk.

Editor: A. D. O'Brien

Infection and Immunity, March 2005, p. 1735-1743, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1735-1743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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