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Infection and Immunity, March 2005, p. 1852-1855, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1852-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Green Fluorescent Protein and Reverse Transcription-PCR To Monitor Candida albicans Agglutinin-Like Sequence Gene Expression in a Murine Model of Disseminated Candidiasis

Clayton B. Green,1 Xiaomin Zhao, and Lois L. Hoyer*

Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois1

Received 30 July 2004/ Returned for modification 1 September 2004/ Accepted 28 October 2004

Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.

* Corresponding author. Mailing address: 2522 VMBSB, 2001 S. Lincoln Ave., Urbana, IL 61802. Phone: (217) 333-5043. Fax: (217) 244-7421. E-mail: lhoyer{at}uiuc.edu.

Editor: T. R. Kozel

Infection and Immunity, March 2005, p. 1852-1855, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1852-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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