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Infection and Immunity, March 2005, p. 1898-1902, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1898-1902.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mycobacterium tuberculosis with Disruption in Genes Encoding the Phosphate Binding Proteins PstS1 and PstS2 Is Deficient in Phosphate Uptake and Demonstrates Reduced In Vivo Virulence

Priska Peirs,1 Philippe Lefèvre,1 Samira Boarbi,1 Xiao-Ming Wang,1 Olivier Denis,1 Martine Braibant,1 Kevin Pethe,2 Camille Locht,2 Kris Huygen,1 and Jean Content1*

Pasteur Institute of Brussels, Brussels, Belgium,1 INSERM U629, Pasteur Institute-Lille, Lille, France2

Received 5 July 2004/ Returned for modification 5 August 2004/ Accepted 2 October 2004

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.

* Corresponding author. Mailing address: Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium. Phone: 32-2-3733416. Fax: 32-2-3733291. E-mail: jcontent{at}pasteur.be.

Editor: F. C. Fang

Infection and Immunity, March 2005, p. 1898-1902, Vol. 73, No. 3
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.3.1898-1902.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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