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Infection and Immunity, April 2005, p. 2327-2335, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2327-2335.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Gene Expression in Porphyromonas gingivalis after Contact with Human Epithelial Cells

Yumiko Hosogi and Margaret J. Duncan*

Department of Molecular Genetics, The Forsyth Institute, Boston, Massachusetts

Received 14 September 2004/ Returned for modification 8 November 2004/ Accepted 15 December 2004

Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set of P. gingivalis genes would be consistently up-regulated during infection of HEp-2 human epithelial cells. P. gingivalis genome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces in P. gingivalis stress-responsive pathways that promote the survival of the bacterium.

* Corresponding author. Mailing address: Department of Molecular Genetics, The Forsyth Institute, 140 Fenway, Boston, MA 02115. Phone: (617) 892-8344. Fax: (617) 262-4021. E-mail: mduncan{at}forsyth.org.

Editor: J. B. Bliska

Infection and Immunity, April 2005, p. 2327-2335, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2327-2335.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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