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Infection and Immunity, April 2005, p. 2387-2399, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2387-2399.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Haemophilus ducreyi Outer Membrane Determinants, Including DsrA, Define Two Clonal Populations

Catherine Dinitra White,1,{dagger} Isabelle Leduc,1,{dagger} Bonnie Olsen, Chrystina Jeter,1 Chavala Harris,1 and Christopher Elkins1,2*

Departments of Medicine,1 Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina2

Received 7 June 2004/ Returned for modification 12 July 2004/ Accepted 17 November 2004

The Haemophilus ducreyi outer membrane component DsrA (for ducreyi serum resistance A) is necessary for complete resistance to normal human serum (NHS). When DsrA expression in 19 temporally and geographically diverse clinical isolates of H. ducreyi was examined by Western blotting, 5 of the strains expressed a different immunotype of the DsrA protein (DsrAII) than the well-characterized prototypical strain 35000HP (DsrAI). The predicted DsrA proteins expressed by the DsrAII strains were 100% identical to each other but only 48% identical to that of strain 35000HP. In addition to the DsrAII protein, class II strains also expressed variant forms of other outer membrane proteins (OMPs) including NcaA (necessary for collagen adhesion A), DltA (ducreyi lectin A), Hlp (H. ducreyi lipoprotein), major OMP, and/or OmpA2 (for OMP A2) and synthesized a distinct, faster-migrating lipooligosaccharide. Based on these data, strains expressing DsrAI were termed class I, and those expressing DsrAII were termed class II. Expression of dsrAII from strain CIP 542 ATCC in the class I dsrAI mutant FX517 (35000HP background), which does not express a DsrA protein, rendered this strain resistant to 50% NHS. This demonstrates that DsrAII protein is also critical to serum resistance. Taken together, these results indicate that there are two clonal populations of H. ducreyi. The implications of two classes of H. ducreyi strains differing in important antigenic outer membrane components are discussed.

* Corresponding author. Mailing address: Departments of Medicine and Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599. Phone: (919) 843-5521. Fax: (919) 843-1015. E-mail: chriselk{at}med.unc.edu.

Editor: D. L. Burns

{dagger} C.D.W. and I.L. contributed equally to this work and therefore should be considered co-first authors.

Infection and Immunity, April 2005, p. 2387-2399, Vol. 73, No. 4
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.4.2387-2399.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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