This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beswick, E. J.
Right arrow Articles by Reyes, V. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beswick, E. J.
Right arrow Articles by Reyes, V. E.

 Previous Article  |  Next Article 

Infection and Immunity, May 2005, p. 2736-2743, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2736-2743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori Binds to CD74 on Gastric Epithelial Cells and Stimulates Interleukin-8 Production

Ellen J. Beswick,1 David A. Bland,2 Giovanni Suarez,1 Carlos A. Barrera,3 Xuejung Fan,1 and Victor E. Reyes1,2*

Departments of Pediatrics,1 Pathology,3 Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 775552

Received 13 October 2004/ Returned for modification 29 November 2004/ Accepted 21 December 2004

The pathogenesis associated with Helicobacter pylori infection requires consistent contact with the gastric epithelium. Although several cell surface receptors have been suggested to play a role in adhesion, the bacterium-host interactions that elicit host responses are not well defined. This study investigated the interaction of H. pylori with the class II major histocompatibility complex (MHC)-associated invariant chain (Ii; CD74), which was found to be highly expressed by gastric epithelial cells. Bacterial binding was increased when CD74 surface expression was increased by gamma interferon (IFN-{gamma}) treatment or by fibroblast cells transfected with CD74, while binding was decreased by CD74 blocking antibodies, enzyme cleavage of CD74, and CD74-coated bacteria. H. pylori was also shown to bind directly to affinity-purified CD74 in the absence of class II MHC. Cross-linking of CD74 and the engagement of CD74 were verified to stimulate IL-8 production by unrelated cell lines expressing CD74 in the absence of class II MHC. Increased CD74 expression by cells increased IL-8 production in response to H. pylori, and agents that block CD74 decreased these responses. The binding of H. pylori to CD74 presents a novel insight into an initial interaction of H. pylori with the gastric epithelium that leads to upregulation of inflammatory responses.

* Corresponding author. Mailing address: Children's Hospital, Room 2.300, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555. Phone: (409) 772-3897. Fax: (409) 772-1761. E-mail: vreyes{at}utmb.edu.

Editor: J. B. Bliska

Infection and Immunity, May 2005, p. 2736-2743, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.2736-2743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stein, R., Smith, M. R., Chen, S., Zalath, M., Goldenberg, D. M. (2009). Combining Milatuzumab with Bortezomib, Doxorubicin, or Dexamethasone Improves Responses in Multiple Myeloma Cell Lines. Clin. Cancer Res. 15: 2808-2817 [Abstract] [Full Text]  
  • Beswick, E. J., Reyes, V. E. (2008). Macrophage Migration Inhibitory Factor and Interleukin-8 Produced by Gastric Epithelial Cells during Helicobacter pylori Exposure Induce Expression and Activation of the Epidermal Growth Factor Receptor. Infect. Immun. 76: 3233-3240 [Abstract] [Full Text]  
  • Stein, R., Mattes, M. J., Cardillo, T. M., Hansen, H. J., Chang, C.-H., Burton, J., Govindan, S., Goldenberg, D. M. (2007). CD74: A New Candidate Target for the Immunotherapy of B-Cell Neoplasms. Clin. Cancer Res. 13: 5556s-5563s [Abstract] [Full Text]  
  • Binsky, I., Haran, M., Starlets, D., Gore, Y., Lantner, F., Harpaz, N., Leng, L., Goldenberg, D. M., Shvidel, L., Berrebi, A., Bucala, R., Shachar, I. (2007). IL-8 secreted in a macrophage migration-inhibitory factor- and CD74-dependent manner regulates B cell chronic lymphocytic leukemia survival. Proc. Natl. Acad. Sci. USA 104: 13408-13413 [Abstract] [Full Text]  
  • Lu, H., Wu, J. Y., Beswick, E. J., Ohno, T., Odenbreit, S., Haas, R., Reyes, V. E., Kita, M., Graham, D. Y., Yamaoka, Y. (2007). Functional and Intracellular Signaling Differences Associated with the Helicobacter pylori AlpAB Adhesin from Western and East Asian Strains. J. Biol. Chem. 282: 6242-6254 [Abstract] [Full Text]  
  • Kobayashi, M., Lee, H., Schaffer, L., Gilmartin, T. J., Head, S. R., Takaishi, S., Wang, T. C., Nakayama, J., Fukuda, M. (2007). A Distinctive Set of Genes Is Upregulated During the Inflammation-Carcinoma Sequence in Mouse Stomach Infected by Helicobacter felis. J. Histochem. Cytochem. 55: 263-274 [Abstract] [Full Text]  
  • Starlets, D., Gore, Y., Binsky, I., Haran, M., Harpaz, N., Shvidel, L., Becker-Herman, S., Berrebi, A., Shachar, I. (2006). Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival. Blood 107: 4807-4816 [Abstract] [Full Text]  
  • Beswick, E. J., Pinchuk, I. V., Suarez, G., Sierra, J. C., Reyes, V. E. (2006). Helicobacter pylori CagA-Dependent Macrophage Migration Inhibitory Factor Produced by Gastric Epithelial Cells Binds to CD74 and Stimulates Procarcinogenic Events.. J. Immunol. 176: 6794-6801 [Abstract] [Full Text]  
  • Beswick, E. J., Pinchuk, I. V., Minch, K., Suarez, G., Sierra, J. C., Yamaoka, Y., Reyes, V. E. (2006). The Helicobacter pylori Urease B Subunit Binds to CD74 on Gastric Epithelial Cells and Induces NF-{kappa}B Activation and Interleukin-8 Production. Infect. Immun. 74: 1148-1155 [Abstract] [Full Text]  
  • Beswick, E. J., Das, S., Pinchuk, I. V., Adegboyega, P., Suarez, G., Yamaoka, Y., Reyes, V. E. (2005). Helicobacter pylori-Induced IL-8 Production by Gastric Epithelial Cells Up-Regulates CD74 Expression. J. Immunol. 175: 171-176 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»