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Infection and Immunity, May 2005, p. 3124-3127, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3124-3127.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Laccase Expression in Murine Pulmonary Cryptococcus neoformans Infection

Javier Garcia-Rivera,1 Stephanie C. Tucker,1 Marta Feldmesser,1,2 Peter R. Williamson,3 and Arturo Casadevall1,2*

Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York,1 Division of Infectious Disease, Albert Einstein College of Medicine, Bronx, New York,2 Division of Infectious Disease, University of Illinois at Chicago College of Medicine, Chicago, Illinois3

Received 27 October 2004/ Returned for modification 3 December 2004/ Accepted 12 January 2005

Cryptococcus neoformans laccase expression during murine infection was investigated in lung tissue by immunohistochemistry and immunogold electron microscopy. Laccase was detected in the fungal cell cytoplasm, cell wall, and capsule in vivo. The amount of laccase found in different sites varied as a function of the time of infection.

* Corresponding author. Mailing address: Division of Infectious Disease, Albert Einstein College of Medicine, 1300 Morris Park Ave., Golding 701, Bronx, NY 10461. Phone: (718) 430-3665. Fax: (718) 430-8701. E-mail: casadeva{at}aecom.yu.edu.

Editor: T. R. Kozel

Infection and Immunity, May 2005, p. 3124-3127, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3124-3127.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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