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Infection and Immunity, May 2005, p. 3152-3159, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3152-3159.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Construction and Characterization of Listeria monocytogenes Mutants with In-Frame Deletions in the Response Regulator Genes Identified in the Genome Sequence

Tatjana Williams,{dagger} Susanne Bauer,{dagger} Dagmar Beier, and Michael Kuhn*

Lehrstuhl für Mikrobiologie, Theodor Boveri-Institut für Biowissenschaften der Universität Würzburg, 97074 Würzburg, Germany

Received 2 September 2004/ Returned for modification 18 October 2004/ Accepted 22 December 2004

Two-component systems are widely distributed in prokaryotes where they control gene expression in response to diverse stimuli. To study the role of the sixteen putative two-component systems of Listeria monocytogenes systematically, in frame deletions were introduced into 15 out of the 16 response regulator genes and the resulting mutants were characterized. With one exception the deletion of the individual response regulator genes has only minor effects on in vitro and in vivo growth of the bacteria. The mutant carrying a deletion in the ortholog of the Bacillus subtilis response regulator gene degU showed a clearly reduced virulence in mice, indicating that DegU is involved in the regulation of virulence-associated genes.

* Corresponding author. Mailing address: Kompetenzzentrum PathoGenoMik, Theodor Boveri-Institut für Biowissenschaften der Universität Würzburg Biozentrum, Am Hubland, 97074 Würzburg, Germany. Phone: (49)-931-8884988. Fax: (49)-931-8884602. E-mail: kuhn{at}biozentrum.uni-wuerzburg.de.

Editor: D. L. Burns

{dagger} Both authors contributed equally.

Infection and Immunity, May 2005, p. 3152-3159, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3152-3159.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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