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Infection and Immunity, May 2005, p. 3188-3191, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3188-3191.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Conversion of Staphylococcus epidermidis Strains from Commensal to Invasive by Expression of the ica Locus Encoding Production of Biofilm Exopolysaccharide

Hualin Li,1 Lin Xu,1 Jianping Wang,1 Yumei Wen,1 Cuong Vuong,2 Michael Otto,2 and Qian Gao1*

Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China,1 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th Street, Hamilton, Montana 598402

Received 30 June 2004/ Returned for modification 14 September 2004/ Accepted 30 December 2004

To test if biofilm formation in Staphylococcus epidermidis is dependent on the polysaccharide intercellular adhesin, whose biosynthesis is driven by the ica locus, a plasmid containing the ica locus was transferred to three ica-negative strains. Using in vitro biofilm assays and a rat central venous catheter infection model, we confirmed the importance of the ica locus for biofilm production and pathogenesis of S. epidermidis.

* Corresponding author. Mailing address: 138 Yi Xue Yuan Road, Shanghai, 200032, PR China. Phone: 86-21-6417-4578. Fax: 86-21-54237195. E-mail: qiangao{at}shmu.edu.cn.

Editor: V. J. DiRita

Infection and Immunity, May 2005, p. 3188-3191, Vol. 73, No. 5
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.5.3188-3191.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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