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Infection and Immunity, June 2005, p. 3294-3300, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3294-3300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

An RNA Vaccine Based on Recombinant Semliki Forest Virus Particles Expressing the Cu,Zn Superoxide Dismutase Protein of Brucella abortus Induces Protective Immunity in BALB/c Mice

Angel A. Oñate,1* Gabriel Donoso,1 Gustavo Moraga-Cid,1 Hugo Folch,2 Sandra Céspedes,1 and Edilia Andrews1

Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción,1 Department of Immunology, Faculty of Medicine, Universidad Austral, Valdivia, Chile2

Received 6 October 2004/ Returned for modification 19 November 2004/ Accepted 31 January 2005

We constructed infectious but replication-deficient Semliki Forest virus (SFV) particles carrying recombinant RNA encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). The recombinant SFV particles (SFV-SOD particles) were then evaluated for their ability to induce a T-cell immune response and to protect BALB/c mice against a challenge with B. abortus 2308. Intraperitoneal injection of mice with recombinant SFV-SOD particles did not lead to the induction of SOD-specific antibodies, at least until week 6 after immunization (the end of the experiment). In vitro stimulation of splenocytes from the vaccinated mice with either recombinant Cu,Zn SOD (rSOD) or crude Brucella protein resulted in a T-cell proliferative response and the induction of gamma interferon secretion but not interleukin-4. In addition, the splenocytes exhibited significant levels of cytotoxic T-lymphocyte activity against Brucella-infected cells. The SFV-SOD particles, but not the control virus particles, induced a significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308. These findings indicated that an SFV-based vector carrying the SOD gene has potential for use as a vaccine to induce resistance against B. abortus infections.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, P.O. Box 152-C, Concepción, Chile. Phone: (056)-41-204118. Fax: (056)-41-245975. E-mail: aonate{at}udec.cl.

Editor: J. D. Clements

Infection and Immunity, June 2005, p. 3294-3300, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3294-3300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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