This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendez, S.
Right arrow Articles by Hotez, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendez, S.
Right arrow Articles by Hotez, P. J.

 Previous Article  |  Next Article 

Infection and Immunity, June 2005, p. 3402-3407, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3402-3407.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Host Cytokine Production, Lymphoproliferation, and Antibody Responses during the Course of Ancylostoma ceylanicum Infection in the Golden Syrian Hamster

Susana Mendez,1* Jesus G. Valenzuela,2 Wenhui Wu,1 and Peter J. Hotez1

Department of Microbiology and Tropical Medicine, The George Washington University Medical Center and The Sabin Vaccine Institute, 2300 Eye Street NW, Washington, DC 20037,1 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 208522

Received 21 December 2004/ Accepted 7 February 2005

The Syrian Golden hamster (Mesocricetus auratus) has been used to model infections with the hookworm Ancylostoma ceylanicum. New molecular immunological reagents to measure cellular immune responses in hamsters were developed and used to determine the impact of A. ceylanicum hookworm infection on host cytokine responses and lymphoproliferation. Initial larval infection with 100 third-stage A. ceylanicum larvae resulted in predominant Th1 responses (upregulation of proinflammatory cytokines) that lasted for the duration of larval migration and continued up to 14 days postinfection (prepatency). Subsequently, development of larvae into egg-laying adult hookworms (patency) coincided with a switch to Th2 predominant responses (interleukin-4 [IL-4]) as well as a marked increase in IL-10 production. This switch also concurred with reduced host lymphoproliferative responses to hookworm antigens. The findings demonstrate a similarity in immune responses between hamsters and humans infected with hookworms, suggesting that hamsters will be a useful animal model species for examining host immunity to human hookworm infections.

* Corresponding author. Mailing address: Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Ross Hall, Rm 726, 2300 Eye St. NW, Washington, DC 20037. Phone: (202) 994-2150. Fax: (202) 841-2913. E-mail: mtmsxm{at}gwumc.edu.

Editor: J. L. Flynn

Infection and Immunity, June 2005, p. 3402-3407, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3402-3407.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dondji, B., Bungiro, R. D., Harrison, L. M., Vermeire, J. J., Bifulco, C., McMahon-Pratt, D., Cappello, M. (2008). Role for Nitric Oxide in Hookworm-Associated Immune Suppression. Infect. Immun. 76: 2560-2567 [Abstract] [Full Text]  
  • Rama Iniguez, S., Dea-Ayuela, M. A., Sanchez-Brunete, J. A., Torrado, J. J., Alunda, J. M., Bolas-Fernandez, F. (2006). Real-Time Reverse Transcription-PCR Quantification of Cytokine mRNA Expression in Golden Syrian Hamster Infected with Leishmania infantum and Treated with a New Amphotericin B Formulation.. Antimicrob. Agents Chemother. 50: 1195-1201 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»