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Infection and Immunity, June 2005, p. 3415-3421, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3415-3421.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of Staphylococcus aureus Global Regulators sae and {sigma}B in Virulence Gene Expression during Device-Related Infection

Christiane Goerke,1* Ursula Fluckiger,2 Andrea Steinhuber,2 Vittoria Bisanzio,1 Martina Ulrich,1 Markus Bischoff,3 Joseph M. Patti,4 and Christiane Wolz1

Institute for Medical Microbiology and Hygiene, University Hospital Tübingen, Tübingen, Germany,1 Division of Infectious Diseases, University Hospitals Basel, Basel,2 Department of Medical Microbiology, University of Zurich, Zurich, Switzerland,3 Inhibitex, Inc., Alpharetta, Georgia4

Received 14 September 2004/ Returned for modification 8 December 2004/ Accepted 19 January 2005

The ability of Staphylococcus aureus to adapt to different environments is due to a regulatory network comprising several loci. Here we present a detailed study of the interaction between the two global regulators sae and {sigma}B of S. aureus and their influence on virulence gene expression in vitro, as well as during device-related infection. The expression of sae, asp23, hla, clfA, coa, and fnbA was determined in strain Newman and its isogenic saeS/R and sigB mutants by Northern analysis and LightCycler reverse transcription-PCR. There was no indication of direct cross talk between the two regulators. sae had a dominant effect on target gene expression during device-related infection. {sigma}B seemed to be less active throughout the infection than under induced conditions in vitro.

* Corresponding author. Mailing address: Institut für Med. Mikrobiologie und Hygiene, Universitätsklinikum Tübingen, Wilhelmstrasse 31, D-72074 Tübingen, Germany. Phone: 49-7071-2980187. Fax: 49-7071-293011. E-mail: christiane.goerke{at}uni-tuebingen.de.

Editor: V. J. DiRita

Infection and Immunity, June 2005, p. 3415-3421, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3415-3421.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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