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Infection and Immunity, June 2005, p. 3471-3478, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3471-3478.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Surface Antigens of Moraxella catarrhalis as Targets of Human Serum Antibody Responses in Chronic Obstructive Pulmonary Disease

Timothy F. Murphy,1,3,4* Aimee L. Brauer,1,4 Christoph Aebi,5,6 and Sanjay Sethi2,4

Division of Infectious Diseases,1 Division of Pulmonary and Critical Care Medicine, Department of Medicine,2 Department of Microbiology, University at Buffalo, State University of New York,3 VA Western New York Healthcare System, Buffalo, New York,4 Institute for Infectious Diseases,5 Department of Pediatrics, University of Bern, Bern, Switzerland6

Received 6 January 2005/ Returned for modification 4 February 2005/ Accepted 14 February 2005

Moraxella catarrhalis is an important respiratory tract pathogen, causing otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease (COPD). Adults with COPD make antibody responses to M. catarrhalis following infection, but little is known about the identity of the antigens to which these antibodies are directed. In this study, 12 serum samples obtained from adults with COPD who had cleared M. catarrhalis from the respiratory tract following infection and who had developed new serum immunoglobulin G (IgG) to their infecting strain were subjected to a series of assays to identify the antigens to which potentially protective antibodies were directed. Sera were adsorbed with intact bacterial cells, and antibodies were eluted from the surfaces of the bacteria. Analysis by flow cytometry established that adsorption and elution effectively detected antibodies specifically directed to surface-exposed epitopes. Immunoblot assays of adsorbed and eluted serum fractions were performed with purified outer membranes and purified lipooligosaccharide of homologous infecting strains and with a series of mutants deficient in expression of individual outer membrane proteins (OMPs). While heterogeneity in antibody responses among individuals was observed, five major OMPs, UspA1, UspA2, Hag, TbpB, and OMP CD, were identified as targets of antibodies to surface epitopes in the majority of adults with COPD who cleared the organism. These results have important implications in understanding human immune responses to M. catarrhalis and in elucidating the elements of a protective immune response.

* Corresponding author. Mailing address: VA Western New York Healthcare System, Medical Research 151, 3495 Bailey Avenue, Buffalo, NY 14215. Phone: (716) 862-7874. Fax: (716) 862-6526. E-mail: murphyt{at}buffalo.edu.

Editor: D. L. Burns

Infection and Immunity, June 2005, p. 3471-3478, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3471-3478.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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