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Infection and Immunity, June 2005, p. 3502-3511, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3502-3511.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Staphylococcus aureus Strains That Express Serotype 5 or Serotype 8 Capsular Polysaccharides Differ in Virulence

Andrew Watts,^1, Danbing Ke,^1,, Qun Wang,¹ Anil Pillay,¹ Anne Nicholson-Weller,² and Jean C. Lee^1*

Channing Laboratory, Brigham and Women's Hospital,¹ Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115²

Received 19 November 2004/ Returned for modification 5 January 2005/ Accepted 25 January 2005

Most isolates of Staphylococcus aureus produce a serotype 5 (CP5) or 8 (CP8) capsular polysaccharide. To investigate whether CP5 and CP8 differ in their biological properties, we created isogenic mutants of S. aureus Reynolds that expressed CP5, CP8, or no capsule. Biochemical analyses of CP5 and CP8 purified from the isogenic S. aureus strains were consistent with published structures. The degree of O acetylation of each polysaccharide was similar, but CP5 showed a greater degree of N acetylation. Mice challenged with the CP5⁺ strain showed a significantly higher bacteremia level than mice challenged with the CP8⁺ strain. Similarly, the CP5⁺ strain survived preferentially in the bloodstream and kidneys of infected mice challenged with a mixed inoculum containing both strains. The enhanced virulence of the CP5⁺ strain in vivo correlated with its greater resistance to in vitro killing in whole mouse blood. Likewise, in vitro opsonophagocytic killing assays with human neutrophils and sera revealed greater survival of the Reynolds (CP5) strain, even though the kinetics of opsonization by C3b and iC3b was similar for both the CP5⁺ and CP8⁺ strains. Electron micrographs demonstrated C3 molecules on the cell wall beneath the capsule layer for both serotype 5 and 8 strains. Purified CP5 and CP8 stimulated a modest oxidative burst in human neutrophils but failed to activate the alternative complement pathway. These results indicate that CP5 and CP8 differ in a number of biological properties, and these differences likely contribute to the relative virulence of serotype 5 and 8 S. aureus in vivo.

Editor: J. N. Weiser

A.W. and D.K. contributed equally to this work.

Present address: Bioniche Therapeutics Research Center, 6100 Royalmount Ave., Montreal, Quebec, Canada H4P 2R2.

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