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Protein Microarray for Profiling Antibody Responses to Yersinia pestis Live Vaccine

Bei Li,^1, Lingxiao Jiang,^2, Qifeng Song,^3,4, Junxin Yang,¹ Zeliang Chen,¹ Zhaobiao Guo,¹ Dongsheng Zhou,¹ Zongmin Du,¹ Yajun Song,¹ Jin Wang,¹ Hongxia Wang,¹ Shouyi Yu,² Jian Wang,³ and Ruifu Yang^1*

Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China,¹ The South Medical University, Guangzhou, China,² Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 100101, China,³ Graduate school of Chinese academy of Sciences, Beijing 100039, China⁴

Received 20 October 2004/ Returned for modification 24 November 2004/ Accepted 6 January 2005

A protein microarray representing 149 Yersinia pestis proteins was developed to profile antibody responses in EV76-immunized rabbits. Antibodies to 50 proteins were detected. There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens.

Editor: J. B. Bliska

B.L, L.J., and Q.S. contributed equally to this work.

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