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Genetic and Functional Analyses of the Actinobacillus actinomycetemcomitans AfeABCD Siderophore-Independent Iron Acquisition System

Eric R. Rhodes, Andrew P. Tomaras, Glen McGillivary, Pamela L. Connerly, and Luis A. Actis^*

Department of Microbiology, Miami University, Oxford, Ohio

Received 8 October 2004/ Returned for modification 3 December 2004/ Accepted 19 January 2005

The Actinobacillus actinomycetemcomitans afeABCD iron transport system, the expression of which is controlled by iron and Fur, was identified in three different isolates. The protein products of this locus are related to bacterial ABC transporters involved in metal transport. Transformation of the Escherichia coli 1017 iron acquisition mutant with a plasmid harboring afeABCD promoted cell growth under iron-chelated conditions. However, insertion disruption of each of the afeABCD coding regions abolished this growth-relieving effect. The replacement of the parental afeA allele with the derivative afeA::EZ::TN<KAN-2> drastically reduced the ability of A. actinomycetemcomitans cells to grow under iron-chelated conditions.

Editor: F. C. Fang

Present address: Columbus Children's Research Institute, Department of Pediatrics, The Ohio State University College of Medicine and Public Health, Columbus, Ohio.

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