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Infection and Immunity, June 2005, p. 3799-3802, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3799-3802.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Combining Suppression Subtractive Hybridization and Microarrays To Map the Intraspecies Phylogeny of Flavobacterium psychrophilum

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington,¹ Department of Fish and Wildlife Resources, University of Idaho, Moscow, Idaho²

Received 15 November 2004/ Returned for modification 6 January 2005/ Accepted 26 January 2005

Reciprocal subtractive libraries were prepared for two strains of Flavobacterium psychrophilum, one virulent and the other avirulent in a trout challenge model. Unique clones were sequenced and their distribution assessed among 34 strains. The analysis showed that F. psychrophilum is composed of two genetic lineages, possibly reflecting host specificity.

Editor: V. J. DiRita

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