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Infection and Immunity, June 2005, p. 3817-3822, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3817-3822.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Immunological Evaluation and Cellular Location Analysis of the TprI Antigen of Treponema pallidum subsp. pallidum

Lorenzo Giacani,1,{dagger} Vittorio Sambri,1*,{dagger} Antonella Marangoni,1 Francesca Cavrini,1 Elisa Storni,1 Manuela Donati,1 Silvia Corona,2 Paolo Lanzarini,2 and Roberto Cevenini1

Sezione di Microbiologia DMCSS, Università degli Studi di Bologna, Bologna, Italy,1 Dipartimento di Malattie Infettive, IRCCS Policlinico San Matteo, Pavia, Italy2

Received 26 October 2004/ Returned for modification 14 December 2004/ Accepted 29 January 2005

The TprI antigen of Treponema pallidum subsp. pallidum is a putative virulence factor predicted to be located in the outer membrane of the syphilis spirochete. In this study, we analyzed the immune response against TprI and its subunits in sera collected both from rabbits experimentally infected with the Nichols strain and from patients with syphilis, showing a different pattern of reactivity toward the antigen in these two groups of samples. The protective ability of recombinant TprI and its hypothetical outer membrane location were also investigated. Although no rabbit was protected after challenge, immunoelectron microscopy results, to be further investigated, were compatible with the outer membrane location of the antigen.

* Corresponding author. Mailing address: Dipartimento di Medicina Clinica, Specialistica e Sperimentale, Sezione di Microbiologia, Policlinico S. Orsola-Malpighi, via Massarenti 9, 40138 Bologna, Italy. Phone: 39 051 4290913. Fax: 39 051 307397. E-mail: vsambri{at}med.unibo.it.

Editor: J. T. Barbieri

{dagger} Lorenzo Giacani and Vittorio Sambri contributed equally to this work.

Infection and Immunity, June 2005, p. 3817-3822, Vol. 73, No. 6
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.6.3817-3822.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Giacani, L., Molini, B., Godornes, C., Barrett, L., Van Voorhis, W., Centurion-Lara, A., Lukehart, S. A. (2007). Quantitative Analysis of tpr Gene Expression in Treponema pallidum Isolates: Differences among Isolates and Correlation with T-Cell Responsiveness in Experimental Syphilis. Infect. Immun. 75: 104-112 [Abstract] [Full Text]  
  • LaFond, R. E., Lukehart, S. A. (2006). Biological Basis for Syphilis. Clin. Microbiol. Rev. 19: 29-49 [Abstract] [Full Text]  


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