This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hunstad, D. A.
Right arrow Articles by Hultgren, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hunstad, D. A.
Right arrow Articles by Hultgren, S. J.

 Previous Article  |  Next Article 

Infection and Immunity, July 2005, p. 3999-4006, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.3999-4006.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Suppression of Bladder Epithelial Cytokine Responses by Uropathogenic Escherichia coli

David A. Hunstad,1 Sheryl S. Justice,2 Chia S. Hung,2 Scott R. Lauer,3 and Scott J. Hultgren2*

Departments of Pediatrics,1 Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110,2 School of Medicine, Saint Louis University, St. Louis, Missouri 631033

Received 17 September 2004/ Returned for modification 4 January 2005/ Accepted 16 February 2005

Urinary tract infections are most commonly caused by uropathogenic strains of Escherichia coli (UPEC), which invade superficial bladder epithelial cells via a type 1 pilus-dependent mechanism. Inside these epithelial cells, UPEC organisms multiply to high numbers to form intracellular bacterial communities, allowing them to avoid immune detection. Bladder epithelial cells produce interleukin-6 (IL-6) and IL-8 in response to laboratory strains of E. coli in vitro. We investigated the ability of UPEC to alter epithelial cytokine signaling by examining the in vitro responses of bladder epithelial cell lines to the cystitis strains UTI89 and NU14. The cystitis strains induced significantly less IL-6 than did the laboratory E. coli strain MG1655 from 5637 and T24 bladder epithelial cells. The cystitis strains also suppressed epithelial cytokine responses to exogenous lipopolysaccharide (LPS) and to laboratory E. coli. We found that insertional mutations in the rfa and rfb operons and in the surA gene all abolished the ability of UTI89 to suppress cytokine induction. The rfa and rfb operons encode LPS biosynthetic genes, while surA encodes a periplasmic cis-trans prolyl isomerase important in the biogenesis of outer membrane proteins. We conclude that, in this in vitro model system, cystitis strains of UPEC have genes encoding factors that suppress proinflammatory cytokine production by bladder epithelial cells.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Washington University School of Medicine, 660 S. Euclid Ave., Campus Box 8230, Saint Louis, MO 63110-1093. Phone: (314) 362-6772. Fax: (314) 362-1998. E-mail: hultgren{at}borcim.wustl.edu.

Editor: A. D. O'Brien

Infection and Immunity, July 2005, p. 3999-4006, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.3999-4006.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Billips, B. K., Schaeffer, A. J., Klumpp, D. J. (2008). Molecular Basis of Uropathogenic Escherichia coli Evasion of the Innate Immune Response in the Bladder. Infect. Immun. 76: 3891-3900 [Abstract] [Full Text]  
  • Himpsl, S. D., Lockatell, C. V., Hebel, J. R., Johnson, D. E., Mobley, H. L. T. (2008). Identification of virulence determinants in uropathogenic Proteus mirabilis using signature-tagged mutagenesis. J Med Microbiol 57: 1068-1078 [Abstract] [Full Text]  
  • Bhushan, S., Tchatalbachev, S., Klug, J., Fijak, M., Pineau, C., Chakraborty, T., Meinhardt, A. (2008). Uropathogenic Escherichia coli Block MyD88-Dependent and Activate MyD88-Independent Signaling Pathways in Rat Testicular Cells. J. Immunol. 180: 5537-5547 [Abstract] [Full Text]  
  • Wiles, T. J., Dhakal, B. K., Eto, D. S., Mulvey, M. A. (2008). Inactivation of Host Akt/Protein Kinase B Signaling by Bacterial Pore-forming Toxins. Mol. Biol. Cell 19: 1427-1438 [Abstract] [Full Text]  
  • Billips, B. K., Forrestal, S. G., Rycyk, M. T., Johnson, J. R., Klumpp, D. J., Schaeffer, A. J. (2007). Modulation of Host Innate Immune Response in the Bladder by Uropathogenic Escherichia coli. Infect. Immun. 75: 5353-5360 [Abstract] [Full Text]  
  • Haugen, B. J., Pellett, S., Redford, P., Hamilton, H. L., Roesch, P. L., Welch, R. A. (2007). In Vivo Gene Expression Analysis Identifies Genes Required for Enhanced Colonization of the Mouse Urinary Tract by Uropathogenic Escherichia coli Strain CFT073 dsdA. Infect. Immun. 75: 278-289 [Abstract] [Full Text]  
  • Justice, S. S., Hunstad, D. A., Seed, P. C., Hultgren, S. J. (2006). Filamentation by Escherichia coli subverts innate defenses during urinary tract infection. Proc. Natl. Acad. Sci. USA 103: 19884-19889 [Abstract] [Full Text]  
  • Justice, S. S., Lauer, S. R., Hultgren, S. J., Hunstad, D. A. (2006). Maturation of Intracellular Escherichia coli Communities Requires SurA.. Infect. Immun. 74: 4793-4800 [Abstract] [Full Text]  
  • Justice, S. S., Hunstad, D. A., Harper, J. R., Duguay, A. R., Pinkner, J. S., Bann, J., Frieden, C., Silhavy, T. J., Hultgren, S. J. (2005). Periplasmic Peptidyl Prolyl cis-trans Isomerases Are Not Essential for Viability, but SurA Is Required for Pilus Biogenesis in Escherichia coli. J. Bacteriol. 187: 7680-7686 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»