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Infection and Immunity, July 2005, p. 4302-4308, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4302-4308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Virulence Attenuation and Live Vaccine Potential of aroA, crp cdt cya, and Plasmid-Cured Mutants of Salmonella enterica Serovar Abortusovis in Mice and Sheep

Sergio Uzzau,1 Gavino Marogna,2 Guido Sisinnio Leori,2 Roy Curtiss III,3 Giuseppe Schianchi,2 Bruce A. D. Stocker,4 and Salvatore Rubino1*

Dipartimento di Scienze Biomediche, Center for Biotechnology Development and Biodiversity Research, Università di Sassari, 07100 Sassari, Italy,1 Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy,2 Department of Biology, Washington University, St. Louis, Missouri 63130,3 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 943054

Received 19 October 2004/ Returned for modification 10 January 2005/ Accepted 16 February 2005

Three live vaccine candidates of Salmonella enterica subspecies I serotype Abortusovis (aroA, cya crp cdt, and plasmid-cured strains) have been developed, and their efficacies in inducing humoral antibodies and protecting against abortion after challenge with wild-type strain SS44 were evaluated in sheep. Following estrus synchronization, animals were immunized 3 weeks after fertilization and boosted once 3 weeks later. Following challenge with wild-type SS44, pregnancy failure of vaccinated ewes was reduced compared to that of nonimmunized controls. Attenuation of each vaccine was also assessed in challenge experiments with nonimmunized pregnant ewes and in BALB/c mice. All three vaccine candidates appear to be safe for use in sheep and provide a model for the development of live vaccine candidates against naturally occurring ovine salmonellosis.

* Corresponding author. Mailing address: Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli Studi di Sassari, Viale S. Pietro, 43B, 07100 Sassari, Italy. Phone: 39.079.228303. Fax: 39.079.212345. E-mail: rubino{at}uniss.it.

Editor: A. D. O'Brien

Infection and Immunity, July 2005, p. 4302-4308, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4302-4308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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