This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Derré, I. Articles by Isberg, R. R. Search for Related Content PubMed PubMed Citation Articles by Derré, I. Articles by Isberg, R. R.

 Previous Article  |  Next Article 

Infection and Immunity, July 2005, p. 4370-4380, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4370-4380.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway

Isabelle Derré^2, and Ralph R. Isberg^1,2*

Howard Hughes Medical Institute,¹ Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, Massachusetts 02111²

Received 14 December 2004/ Returned for modification 17 January 2005/ Accepted 18 February 2005

Legionella pneumophila uses a type IV secretion system to deliver effector molecules into the host cell and establish its replication vacuole. In this study, we investigated the role of LidA, a translocated substrate associated with the surface of the L. pneumophila-containing vacuole. LidA is secreted into the host cell throughout the replication cycle of the bacteria and associates with compartments of the early secretory pathway. When overexpressed in mammalian cells or yeast, LidA interferes with the early secretory pathway, probably via a domain predicted to be rich in coiled-coil structure. Finally, during intracellular replication, the replication vacuoles are in close contact with the endoplasmic reticulum-Golgi intermediate compartment and the Golgi apparatus, suggesting a positive correlation between intracellular growth and association of the vacuole with compartments of the early secretory pathway. We propose that LidA is involved in the recruitment of early secretory vesicles to the L. pneumophila-containing vacuole and that the vacuole associates with the secretory pathway to facilitate this process.

Editor: D. L. Burns

Present address: Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06519.

This article has been cited by other articles:

• Liu, Y., Gao, P., Banga, S., Luo, Z.-Q. (2008). An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors. Proc. Natl. Acad. Sci. USA 105: 9385-9390 [Abstract] [Full Text]  
• Brumell, J. H., Scidmore, M. A. (2007). Manipulation of Rab GTPase Function by Intracellular Bacterial Pathogens. Microbiol. Mol. Biol. Rev. 71: 636-652 [Abstract] [Full Text]  
• Liu, Y., Luo, Z.-Q. (2007). The Legionella pneumophila Effector SidJ Is Required for Efficient Recruitment of Endoplasmic Reticulum Proteins to the Bacterial Phagosome. Infect. Immun. 75: 592-603 [Abstract] [Full Text]  
• Losick, V. P., Isberg, R. R. (2006). NF-{kappa}B translocation prevents host cell death after low-dose challenge by Legionella pneumophila. J. Exp. Med. 203: 2177-2189 [Abstract] [Full Text]  
• VanRheenen, S. M., Luo, Z.-Q., O'Connor, T., Isberg, R. R. (2006). Members of a Legionella pneumophila Family of Proteins with ExoU (Phospholipase A) Active Sites Are Translocated to Target Cells.. Infect. Immun. 74: 3597-3606 [Abstract] [Full Text]