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Infection and Immunity, July 2005, p. 4391-4394, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4391-4394.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of the Distal sarA Promoters in SarA Expression in Staphylococcus aureus

Ambrose L. Cheung and Adhar C. Manna*

Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755

Received 21 January 2005/ Returned for modification 17 February 2005/ Accepted 8 March 2005

The global regulatory locus sarA comprises a 375-bp open reading frame that is driven by three promoters, the proximal P1 and distal P3 and P2 promoters. We mutated the weaker P3 and P2 promoters to ascertain the effect of the change on SarA protein and target gene expression. Our results indicated that the solely active P1 promoter led to a lower SarA protein level, which has an effect on agr transcription and subsequently had corresponding effects on hla, sspA, and spa transcription, probably in both agr-independent and agr-dependent manners.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Dartmouth Medical School, Vail 205, Hanover, NH 03755. Phone: (603) 650-1310. Fax: (603) 650-1362. E-mail: Adhar.C.Manna{at}Dartmouth.edu.

Editor: J. T. Barbieri

Infection and Immunity, July 2005, p. 4391-4394, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4391-4394.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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