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Infection and Immunity, July 2005, p. 4441-4444, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4441-4444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Order of Prime-Boost Vaccination of Neonatal Calves with Mycobacterium bovis BCG and a DNA Vaccine Encoding Mycobacterial Proteins Hsp65, Hsp70, and Apa Is Not Critical for Enhancing Protection against Bovine Tuberculosis

Margot A. Skinner,1,{dagger} D. Neil Wedlock,1 Geoffrey W. de Lisle,1 Michèle M. Cooke,1,{ddagger} Ricardo E. Tascon,2 Jose C. Ferraz,2 Douglas B. Lowrie,2 H. Martin Vordermeier,3 R. Glyn Hewinson,3 and Bryce M. Buddle1*

AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand,1 National Institute for Medical Research, Mill Hill, London, United Kingdom,2 Veterinary Laboratory Agency, Weybridge, Surrey, United Kingdom3

Received 20 December 2004/ Returned for modification 4 February 2005/ Accepted 11 February 2005

Priming neonatal calves at birth with a Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine and boosting with a DNA vaccine consisting of plasmids encoding mycobacterial antigens Hsp65, Hsp70, and Apa or the reverse prime-boost sequence induced similar levels of protection against experimental challenge with Mycobacterium bovis. When M. bovis was isolated from a thoracic lymph node following challenge, the two groups of calves given the prime-boost regimen had significantly lower numbers of M. bovis isolates than those vaccinated with BCG alone. These observations suggest that the exact sequence of administration of a prime-boost vaccination regimen in a neonatal animal model is not critical to the development of immunity.

* Corresponding author. Mailing address: AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand. Phone: 64-4-529 0418. Fax: 64-4-529 0380. E-mail: bryce.buddle{at}agresearch.co.nz.

Editor: F. C. Fang

{dagger} Present address: HortResearch, Mt. Albert, 120 Mt. Albert Road, Private Bag 92 169, Auckland, New Zealand.

{ddagger} Present address: Institute of Veterinary Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.

Infection and Immunity, July 2005, p. 4441-4444, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4441-4444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wedlock, D. N., Denis, M., Painter, G. F., Ainge, G. D., Vordermeier, H. M., Hewinson, R. G., Buddle, B. M. (2008). Enhanced Protection against Bovine Tuberculosis after Coadministration of Mycobacterium bovis BCG with a Mycobacterial Protein Vaccine-Adjuvant Combination but Not after Coadministration of Adjuvant Alone. CVI 15: 765-772 [Abstract] [Full Text]  


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