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Infection and Immunity, August 2005, p. 4539-4547, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4539-4547.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Stimulation of gp91 Phagocytic Oxidase and Reactive Oxygen Species in Neutrophils by an Avirulent Salmonella enterica Serovar Infantis Strain Protects Gnotobiotic Piglets from Lethal Challenge with Serovar Typhimurium Strain F98 without Inducing Intestinal Pathology

Neil Foster,1,{dagger} Scott Hulme,1 Margaret Lovell,1 Katharine Reed,2 and Paul Barrow1*

Institute for Animal Health, Compton Laboratory, Compton RG20 7NN, United Kingdom,1 Department of Clinical Pharmacology, Radcliffe Infirmary, University of Oxford, Oxford OX2 6HE, United Kingdom2

Received 17 September 2004/ Returned for modification 20 December 2004/ Accepted 17 February 2005

Preinoculation of susceptible 5-day-old gnotobiotic piglets with Salmonella enterica serovar Infantis strain 1326/28{Phi}r stimulates neutrophil migration into the intestine, which rapidly protects the pigs against a subsequent (normally lethal) challenge with S. enterica serovar Typhimurium strain F98. Here we show that inoculation with either 1326/28{Phi}r or F98 activated reactive oxygen species (ROS) in neutrophils via NADPH pathways in vivo and in vitro and that the survival of both Salmonella strains was increased if neutrophils were cocultured with the ROS inhibitor N-acetylcysteine (captopril). Neither F98 nor 1326/28{Phi}r significantly increased reactive nitrogen species (RNS) levels in neutrophils isolated from uninfected pigs. Our results indicate the following: (i) rapid protection of highly susceptible gnotobiotic piglets against F98-induced gastroenteritis by preinoculation with 1326/28{Phi}r is likely to be due to stimulation of ROS-producing neutrophils in the intestinal epithelium prior to challenge with the lethal strain; (ii) pathological lesions of the intestine during severe gastroenteritis are not necessarily induced by neutrophil migration per se; and (iii) if neutrophil migration into the intestine is responsible for pathology, then neither increased production of ROS or RNS (in pigs inoculated with the lethal strain) nor reduced production (in protected pigs in which pathological lesions are ameliorated by preinoculation with 1326/28{Phi}r) can account for this phenomenon.

* Corresponding author. Mailing address: Institute for Animal Health, Compton Laboratory, Compton RG20 7NN, United Kingdom. Phone: 44-1635-578411. Fax: 44-1635-577263. E-mail: Paul.barrow{at}bbsrc.ac.uk.

Editor: F. C. Fang

{dagger} Present address: Oral Microbiology and Host Response Group, School of Dental Sciences, Newcastle University Medical School, Newcastle NE2 4BW, United Kingdom.

Infection and Immunity, August 2005, p. 4539-4547, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4539-4547.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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