This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mayr, U. B.
Right arrow Articles by Ignatyev, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mayr, U. B.
Right arrow Articles by Ignatyev, G.

 Previous Article  |  Next Article 

Infection and Immunity, August 2005, p. 4810-4817, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4810-4817.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bacterial Ghosts as an Oral Vaccine: a Single Dose of Escherichia coli O157:H7 Bacterial Ghosts Protects Mice against Lethal Challenge

Ulrike Beate Mayr,1* Christoph Haller,1 Wolfgang Haidinger,1 Alena Atrasheuskaya,3 Eugenij Bukin,3 Werner Lubitz,1,2 and Georgy Ignatyev3

Faculty of Life Science, University of Vienna, Department of Medical/Pharmaceutical Chemistry, Althanstraße 14, UZAII, 2B522, 1090 Vienna, Austria,1 Bird-C GmbH, Schönborngasse 12/12, 1080 Vienna, Austria,2 Institute of Molecular Biology, State Research Centre of Virology and Biotechnology "Vector," 630559 Novosibirsk Region, Koltsovo, Russia3

Received 26 November 2004/ Returned for modification 27 January 2005/ Accepted 24 March 2005

Enterohemorrhagic Escherichia coli (EHEC) is a bacterial pathogen that is associated with several life-threatening diseases for humans. The combination of protein E-mediated cell lysis to produce EHEC ghosts and staphylococcal nuclease A to degrade DNA was used for the development of an oral EHEC vaccine. The lack of genetic material in the oral EHEC bacterial-ghost vaccine abolished any hazard of horizontal gene transfer of resistance genes or pathogenic islands to resident gut flora. Intragastric immunization of mice with EHEC ghosts without the addition of any adjuvant induced cellular and humoral immunity. Immunized mice challenged at day 55 showed 86% protection against lethal challenge with a heterologous EHEC strain after single-dose oral immunization and 93.3% protection after one booster at day 28, whereas the controls showed 26.7% and 30% survival, respectively. These results indicate that it is possible to develop an efficacious single-dose oral EHEC bacterial-ghost vaccine.

* Corresponding author. Mailing address: Faculty of Life Science, University of Vienna, Department of Medical/Pharmaceutical Chemistry, Althanstraße 14, UZAII, 2B522, 1090 Vienna, Austria. Phone: 43-1-4277-55115. Fax: 43-1-4277-55120. E-mail: ulrike.beate.mayr{at}univie.ac.at.

Editor: A. D. O'Brien

Infection and Immunity, August 2005, p. 4810-4817, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4810-4817.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»