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Infection and Immunity, August 2005, p. 4955-4959, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4955-4959.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Impaired Neutrophil Migration Associated with Specific Bovine CXCR2 Genotypes

M. Rambeaud and G. M. Pighetti*

Department of Animal Science, The University of Tennessee, Knoxville, Tennessee 37996

Received 8 December 2004/ Returned for modification 17 January 2005/ Accepted 6 April 2005

Bovine mastitis continues to be the most detrimental factor for profitable dairying. Recent research conducted within our laboratory has identified a genetic marker in the CXCR2 gene associated with mastitis susceptibility. The objective of the present study was to evaluate the migratory ability of neutrophils from cows with different CXCR2 +777 genotypes. Neutrophils isolated from peripheral blood of 30 Holstein cows were tested for in vitro migration and adhesion molecule expression. Cows with the CC or GC genotype at CXCR2 +777 showed significantly lower neutrophil migration to recombinant human interleukin-8 (rhIL-8) than cows with the GG genotype (P < 0.05). Cows with the CC genotype at CXCR2 +777 also showed decreased neutrophil migration to zymosan-activated serum compared to these same cows (P < 0.05). Decreased upregulation of CD18 expression was observed after stimulation with rhIL-8 in cows expressing the CXCR2 +777 CC genotype compared to cows expressing the GG genotype (P < 0.05). A similar trend was observed for CD11b (P < 0.10). However, no difference in CD62 downregulation was observed with respect to genotype. These results provide initial evidence for a phenotypic association between a single nucleotide polymorphism and neutrophil function in dairy cows, as well as potential insight into specific mechanisms affected in cows more susceptible to mastitis.

* Corresponding author. Mailing address: 2640 Morgan Circle, 114 McCord Hall, Knoxville, TN 37996. Phone: (865) 974-7225. Fax: (865) 974-3394. E-mail: pighetti{at}utk.edu.

Editor: J. D. Clements

Infection and Immunity, August 2005, p. 4955-4959, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.4955-4959.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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