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Infection and Immunity, August 2005, p. 5039-5047, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5039-5047.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Scrub Typhus Vaccine Candidate Kp r56 Induces Humoral and Cellular Immune Responses in Cynomolgus Monkeys

Suchismita Chattopadhyay,1 Ju Jiang,1 Teik-Chye Chan,1 T. Scott Manetz,2 Chien-Chung Chao,1 Wei-Mei Ching,1,3 and Allen L. Richards1,3*

Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland,1 TherImmune Research Corporation, Gaithersburg, Maryland,2 Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland3

Received 16 December 2004/ Returned for modification 2 March 2005/ Accepted 28 March 2005

A truncated recombinant 56-kDa outer membrane protein of the Karp strain of Orientia tsutsugamushi (Kp r56) was evaluated in cynomolgus monkeys (Macaca fascicularis) for immunogenicity and safety as a vaccine candidate for the prevention of scrub typhus. This recombinant antigen induced strong humoral and cellular immune responses in two monkeys and was found to be well tolerated. Antigen-specific immunoglobulin M (IgM) and IgG were produced to almost maximal levels within 1 week of a single immunization. Peripheral blood mononuclear cells from vaccinated animals showed an induction of antigen-specific proliferation and gamma interferon production. The Kp r56 was not as efficient as infection with live organisms in preventing reinfection but was able to reduce the inflammation produced at the site of challenge. This report describes the results of the first systematic study of the immunogenicity of a recombinant scrub typhus vaccine candidate in a nonhuman primate model.

* Corresponding author. Mailing address: Rickettsial Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910. Phone: (301) 319-7668. Fax: (301) 319-7460. E-mail: RichardsA{at}nmrc.navy.mil.

Editor: J. D. Clements

Infection and Immunity, August 2005, p. 5039-5047, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5039-5047.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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