This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, C.-I
Right arrow Articles by Secombes, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, C.-I
Right arrow Articles by Secombes, C. J.

 Previous Article  |  Next Article 

Infection and Immunity, August 2005, p. 5053-5064, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5053-5064.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a Novel Cathelicidin Gene in the Rainbow Trout, Oncorhynchus mykiss

Chin-I Chang,1,2,{dagger} Olga Pleguezuelos,1,{dagger} Yong-An Zhang,1,3 Jun Zou,1 and Christopher J. Secombes1*

Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen AB24 2TZ, United Kingdom,1 Fisheries Research Institute, Keelung, Taiwan,2 Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China3

Received 24 September 2004/ Returned for modification 15 December 2004/ Accepted 10 March 2005

We report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5' flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I · C) also expressed rtCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.

* Corresponding author. Mailing address: Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Zoology Building, Tillydrone Avenue, Aberdeen, AB24 2TZ, United Kingdom. Phone: 44-1224-272872. Fax: 44-1224-272396. E-mail: c.secombes{at}abdn.ac.uk.

Editor: J. F. Urban, Jr.

{dagger} Chin-I Chang and Olga Pleguezuelos contributed equally to the work.

Infection and Immunity, August 2005, p. 5053-5064, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5053-5064.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Feng, C. Y., Johnson, S. C., Hori, T. S., Rise, M., Hall, J. R., Gamperl, A. K., Hubert, S., Kimball, J., Bowman, S., Rise, M. L. (2009). Identification and analysis of differentially expressed genes in immune tissues of Atlantic cod stimulated with formalin-killed, atypical Aeromonas salmonicida. Physiol. Genomics 37: 149-163 [Abstract] [Full Text]  
  • Yamasaki, K., Schauber, J., Coda, A., Lin, H., Dorschner, R. A., Schechter, N. M., Bonnart, C., Descargues, P., Hovnanian, A., Gallo, R. L. (2006). Kallikrein-mediated proteolysis regulates the antimicrobial effects of cathelicidins in skin. FASEB J. 20: 2068-2080 [Abstract] [Full Text]  
  • Chang, C.-I, Zhang, Y.-A., Zou, J., Nie, P., Secombes, C. J. (2006). Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar). Antimicrob. Agents Chemother. 50: 185-195 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»