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Infection and Immunity, August 2005, p. 5086-5092, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5086-5092.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Heme Transfer from Streptococcal Cell Surface Protein Shp to HtsA of Transporter HtsABC

Mengyao Liu and Benfang Lei*

Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717

Received 29 November 2004/ Returned for modification 20 January 2005/ Accepted 10 March 2005

Human pathogen group A streptococcus (GAS) can take up heme from host heme-containing proteins as a source of iron. Little is known about the heme acquisition mechanism in GAS. We recently identified a streptococcal cell surface protein (designated Shp) and the lipoprotein component (designated HtsA) of an ATP-binding cassette (ABC) transporter made by GAS as heme-binding proteins. In an effort to delineate the molecular mechanism involved in heme acquisition by GAS, heme-free Shp (apo-Shp) and HtsA (apo-HtsA) were used to investigate heme transfer from heme-containing proteins (holo proteins) to the apo proteins. In addition, the interaction between holo-Shp and holo-HtsA was examined using native polyacrylamide gel electrophoresis. Heme was efficiently transferred from holo-Shp to apo-HtsA but not from holo-HtsA to apo-Shp. Apo-Shp acquired heme from human hemoglobin, and holo-Shp and holo-HtsA were able to form a complex, suggesting that Shp actively relays heme from hemoglobin to apo-HtsA. These findings demonstrate for the first time complex formation and directional heme transfer between a cell surface heme-binding protein and the lipoprotein of a heme-specific ABC transporter in gram-positive bacteria.

* Corresponding author. Mailing address: Veterinary Molecular Biology, Montana State University, P.O. Box 173610, Bozeman, MT 59717. Phone: (406) 994-6389. Fax: (406) 994-4303. E-mail: blei{at}montana.edu.

Editor: V. J. DiRita

Infection and Immunity, August 2005, p. 5086-5092, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5086-5092.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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