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Infection and Immunity, August 2005, p. 5222-5228, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5222-5228.2005

Importance of srtA and srtB for Growth of Bacillus anthracis in Macrophages

Steven D. Zink and Drusilla L. Burns*

Laboratory of Respiratory and Special Pathogens, Food and Drug Administration, Bethesda, Maryland 20892

Received 10 January 2005/ Returned for modification 20 February 2005/ Accepted 10 March 2005

We examined the effect of mutation of two sortase genes of Bacillus anthracis, srtA and srtB, on the ability of the bacterium to grow in J774A.1 cells, a mouse macrophage-like cell line. While disruption of either srtA or srtB had no effect on the ability of the bacteria to grow in rich culture media, mutations in each of these genes dramatically attenuated growth of the bacterium in J774A.1 cells. Complementation of the mutation restored the ability of bacteria to grow in the cells. Since the initial events in inhalation anthrax are believed to be uptake of B. anthracis spores by alveolar macrophages followed by germination of the spores and growth of the bacteria within the macrophages, these results suggest that two sortases of B. anthracis may be critical in the early stages of inhalation anthrax.

* Corresponding author. Mailing address: CBER, FDA HFM-434, 8800 Rockville Pike, Bethesda, MD 20892. Phone: (301) 402-3553. Fax: (301) 402-2776. E-mail: burns{at}cber.fda.gov.

Editor: J. D. Clements

Infection and Immunity, August 2005, p. 5222-5228, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5222-5228.2005




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