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Infection and Immunity, August 2005, p. 5233-5237, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5233-5237.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mapping of the Binding Site for Mannheimia haemolytica Leukotoxin within Bovine CD18

T. Dileepan, M. S. Kannan, B. Walcheck, P. Thumbikat, and S. K. Maheswaran*

Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota

Received 9 November 2004/ Returned for modification 30 November 2004/ Accepted 4 April 2005

To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological activity within bovine CD18, bovine x human CD18 chimeric constructs were generated and coexpressed with bovine CD11a in K562 cells. Studies with the chimeric leukocyte function-associated antigen 1 transductants demonstrate that the site required for LktA binding and biological effects resides within amino acid residues 500 and 600 of the extracellular region of bovine CD18.

* Corresponding author. Mailing address: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108. Phone: (612) 625-6264. Fax: (612) 625-5203. E-mail: mahes001{at}umn.edu.

Editor: J. T. Barbieri

Infection and Immunity, August 2005, p. 5233-5237, Vol. 73, No. 8
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.8.5233-5237.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Dileepan, T., Kachlany, S. C., Balashova, N. V., Patel, J., Maheswaran, S. K. (2007). Human CD18 Is the Functional Receptor for Aggregatibacter actinomycetemcomitans Leukotoxin. Infect. Immun. 75: 4851-4856 [Abstract] [Full Text]  
  • Dassanayake, R. P., Maheswaran, S. K., Srikumaran, S. (2007). Monomeric Expression of Bovine {beta}2-Integrin Subunits Reveals Their Role in Mannheimia haemolytica Leukotoxin-Induced Biological Effects. Infect. Immun. 75: 5004-5010 [Abstract] [Full Text]  


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