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Infection and Immunity, September 2005, p. 5291-5300, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5291-5300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Novel Sialic Acid Transporter of Haemophilus influenzae{dagger}

Simon Allen,1 Anthony Zaleski,2 Jason W. Johnston,2 Bradford W. Gibson,1,3 and Michael A. Apicella2*

Buck Institute for Age Research, 8001 Redwood Blvd., Novato, California 94945,1 Department of Microbiology, University of Iowa, Iowa City, Iowa 52242,2 Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-04463

Received 7 February 2005/ Returned for modification 30 March 2005/ Accepted 24 May 2005

Nontypeable Haemophilus influenzae is an opportunistic pathogen and a common cause of otitis media in children and of chronic bronchitis and pneumonia in patients with chronic obstructive pulmonary disease. The lipooligosaccharides, a major component of the outer membrane of H. influenzae, play an important role in microbial virulence and pathogenicity. N-Acetylneuraminic acid (sialic acid) can be incorporated into the lipooligosaccharides as a terminal nonreducing sugar. Although much of the pathway of sialic acid incorporation into lipooligosaccharides is understood, the transporter responsible for N-acetylneuraminic acid uptake in H. influenzae has yet to be characterized. In this paper we demonstrate that this transporter is a novel sugar transporter of the tripartite ATP-independent periplasmic transporter family. In the absence of this transporter, H. influenzae cannot incorporate sialic acid into its lipooligosaccharides, making the organism unable to survive when exposed to human serum and causing reduced viability in biofilm growth.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7807. Fax: (319) 335-9006. E-mail: michael-apicella{at}uiowa.edu.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: J. N. Weiser

Infection and Immunity, September 2005, p. 5291-5300, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5291-5300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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