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Infection and Immunity, September 2005, p. 5524-5529, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5524-5529.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a New Virulence Factor, BvfA, in Brucella suis

Jean-Philippe Lavigne,1,2 Gilles Patey,1 Felix J. Sangari,3 Gisèle Bourg,1 Michel Ramuz,1 David O'Callaghan,1* and Sylvie Michaux-Charachon1,2

Institut National de la Santé et de la Recherche Médicale U431, UFR Médecine, CS83021, Avenue Kennedy, 30908 Nîmes Cedex 02, France,1 Laboratoire de Bactériologie-Virologie, CHU de Nîmes, Groupe Hospitalo-Universitaire de Carémeau, Place du Professeur Robert Debré, 30029 Nîmes Cedex 09, France,2 Departmento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al Centro de Investigaciones Biológicas, Cardenal Herrera Oria s/n, 39011-Santander, Spain3

Received 7 March 2005/ Returned for modification 13 April 2005/ Accepted 2 May 2005

We report the identification of BvfA (for Brucella virulence factor A), a small periplasmic protein unique to the genus Brucella, which is essential for the virulence of Brucella suis. A BvfA knockout mutant was highly attenuated both in in vitro macrophage infection assays and in vivo in the murine model of brucellosis. Fluorescence-activated cell sorting analysis with green fluorescent protein fusions showed that the expression of bvfA is induced within macrophages by phagosome acidification and coregulated with the B. suis virB operon, suggesting that it too may play a role in the establishment of the intracellular replication niche.

* Corresponding author. Mailing address: Institut National de la Santé et de la Recherche Médicale, U431, Faculté de Médecine, Avenue Kennedy, 30900 Nimes, France. Phone: 33-4-66-02-81-49. Fax: 33-4-66-02-81-48. E-mail: david.ocallaghan{at}univ-montp1.fr.

Editor: D. L. Burns

Infection and Immunity, September 2005, p. 5524-5529, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5524-5529.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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