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Infection and Immunity, September 2005, p. 5540-5546, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5540-5546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Novel Staphylococcal Enterotoxin-Like Toxin Type P

Katsuhiko Omoe,1* Ken'ichi Imanishi,2 Dong-Liang Hu,3 Hidehito Kato,2 Yoshitaku Fugane,1 Yohei Abe,1 Shoji Hamaoka,1 Yutaka Watanabe,1 Akio Nakane,3 Takehiko Uchiyama,2 and Kunihiro Shinagawa1

Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550,1 Department of Microbiology and Immunology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666,2 Department of Bacteriology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan3

Received 1 February 2005/ Returned for modification 20 March 2005/ Accepted 16 May 2005

We investigated the biological properties of a novel staphylococcal enterotoxin (SE)-like toxin type P (SElP). SElP induced a substantial proliferative response and the production of cytokines interleukin-2, gamma interferon, tumor necrosis factor alpha, and interleukin-4 from human T cells when administered at a concentration of 0.4 pM (0.01 ng/ml) or more. The expression of major histocompatibility complex class II molecules on accessory cells was required for T-cell stimulation by SElP. SElP selectively stimulated a vast number of human T cells bearing receptors Vß 5.1, 6, 8, 16, 18, and 21.3. These results indicated that SElP acts as a superantigen. SElP proved to be emetic in the house musk shrew emetic assay, although at a relatively high dose (50 to 150 µg/animal). A quantitative assay of SElP production with 30 Staphylococcus aureus strains harboring selp showed that 60% of these strains produced significant amounts of SElP in vitro. All 10 strains carrying seb and selp produced SEB but not SElP, suggesting the inactivation of the selp locus in S. aureus strains with a particular se gene constitution.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550, Japan. Phone: 81-19-621-6221. Fax: 81-19-621-6223. E-mail: omo{at}iwate-u.ac.jp.

Editor: J. T. Barbieri

Infection and Immunity, September 2005, p. 5540-5546, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5540-5546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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