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Infection and Immunity, September 2005, p. 5762-5766, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5762-5766.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Neisseria meningitidis Lactate Permease Is Required for Nasopharyngeal Colonization

Rachel M. Exley,1 Linda Goodwin,2 Eva Mowe,1 Jonathan Shaw,2 Harry Smith,3 Robert C. Read,2 and Christoph M. Tang1*

The Centre for Molecular Microbiology and Infection, Flowers Building, Imperial College London, Armstrong Rd., London SW7 2AZ, United Kingdom,1 Division of Genomic Medicine, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom,2 The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom3

Received 11 February 2005/ Returned for modification 29 March 2005/ Accepted 10 May 2005

Neisseria meningitidis is a human specific pathogen that is part of the normal nasopharyngeal flora. Little is known about the metabolic constraints on survival of the meningococcus during colonization of the upper airways. Here we show that glucose and lactate, both carbon energy sources for meningococcal growth, are present in millimolar concentrations within nasopharyngeal tissue. We used a mutant defective for the uptake of lactate (C311{Delta}lctP) to investigate the contribution of this energy source during colonization. Explants of nasopharyngeal tissue were inoculated with the wild-type strain (C311) and C311{Delta}lctP; the mutant was recovered at significantly lower levels (P = 0.01) than C311 18 h later. This defect was not due to changes in the expression of adhesins or initial adhesion in C311{Delta}lctP to epithelial cells. Instead, lactate appears to be important energy source for the bacterium during colonization and is necessary for growth of the bacterium in nasopharyngeal tissue. Studies with other strains defective for the uptake of specific nutrients should provide valuable information about the environment in which N. meningitidis persists during carriage.

* Corresponding author. Mailing address: The Centre for Molecular Microbiology and Infection, Flowers Building, Imperial College London, Armstrong Rd., London SW7 2AZ, United Kingdom. Phone: 44-20-7594-3072. Fax: 44-20-7594-3076. E-mail: c.tang{at}imperial.ac.uk.

Editor: V. J. DiRita

Infection and Immunity, September 2005, p. 5762-5766, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.5762-5766.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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