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Infection and Immunity, September 2005, p. 6179-6182, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.6179-6182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mannheimia (Pasteurella) haemolytica Leukotoxin Binding Domain Lies within Amino Acids 1 to 291 of Bovine CD18

R. S. Gopinath, T. C. Ambagala, M. S. Deshpande, R. O. Donis,{dagger} and S. Srikumaran*

Department of Veterinary and Biomedical Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska 68583-0905

Received 5 January 2005/ Returned for modification 5 April 2005/ Accepted 20 April 2005

Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia (Pasteurella) haemolytica. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.

* Corresponding author. Present address: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040. Phone: (509) 335-4572. Fax: (509) 335-8529. E-mail: ssrikumaran{at}vetmed.wsu.edu.

Editor: A. D. O'Brien

{dagger} Present address: Influenza Branch, DVRD, NCID, Centers for Disease Control and Prevention, Mail Stop G-16, 1600 Clifton Road, Atlanta, GA 30333.

Infection and Immunity, September 2005, p. 6179-6182, Vol. 73, No. 9
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.9.6179-6182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shanthalingam, S., Srikumaran, S. (2009). Intact signal peptide of CD18, the {beta}-subunit of {beta}2-integrins, renders ruminants susceptible to Mannheimia haemolytica leukotoxin. Proc. Natl. Acad. Sci. USA 106: 15448-15453 [Abstract] [Full Text]  
  • Atapattu, D. N., Czuprynski, C. J. (2007). Mannheimia haemolytica Leukotoxin Binds to Lipid Rafts in Bovine Lymphoblastoid Cells and Is Internalized in a Dynamin-2- and Clathrin-Dependent Manner. Infect. Immun. 75: 4719-4727 [Abstract] [Full Text]  
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